Memberships & activities in Scientific and Professional Activities
- Invited Member Pulmonary Vascular Research Institute, UK
- Executive Council, Indian Society for Atherosclerosis Research,
- Life Member, Indian Society for Atherosclerosis Research
- Life Member, International Atherosclerotic Society
- Life Member, International Society for Heart Research (India Chapter)
- Life Member, International Academy of Cardiovascular Sciences
- Life Member, Indian Society for Hematology & Transfusion Medicine
- Member, Society for Vascular Medicine and Biology
- Member, North American Vascular Biology Organization
- Member, American Heart Association (2003-2009)
Editorial /Reviewer Roles for Scientific Journals:
- Blood
- Blood Advances
- International Journal of Hematology Research
- Frontiers in Chemical Sciences
- Journal Of Thrombosis & Haemostasis
- Thrombosis Research
- High Altitude Medicine & Biology
- Scientific Reports
- American Journal of Hypertension
- Journal of Hypertension
- PLoS ONE
- International Journal of Biochemistry & Cell Biology
- Pharmaceutical Biology
- Human Toxicology
- Microbes & Infection
Research Overview
Dr. Ashraf has focused his research that was of urgent need and challenge for nation and scientific community to elucidate the blood clotting problem inflicting our soldiers that are positioned at high altitude defending our borders. He remains a pioneer in the field of high altitude thrombosis and has done seminal work in resolving the mystery of blood clotting on exposure to hypoxia.
Amongst the major outcomes of his translational research, the landmark work was internationally recognized and published in prominent journal ‘Blood’ in 2014, received accolade by an Editorial. The study revealed a novel cause for blood clot formation at altitudes and proposed ‘Calpain’ as a novel biomarker (IPO: 733/DEL/2014) for early diagnosis; thereby providing timely diagnosis to Indian Army Jawans posted at Siachen Glaciers defending our borders.
Dr. Ashraf study has contributed enormously in understanding the etiopathology of clot formation at altitudes, for the development of possible drug targets. He elucidated previously unknown cause of thrombosis at altitudes is centrally regulated by a complex network of coagulatory and NLRP3-inflammasome mediated inflammatory responses. His research established (PNAS, 2017) that, hypoxia-inducible Factor-(HIF)-1α, (Nobel prize for Medicine 2019) a protein complex plays an integral role in the body's response to low-oxygen, activates an inflammatory complex inflammasome-NLRP3 and releases inflammatory cytokines like IL-18 and IL-1β, which eventually causes blood clotting. This novel finding was highly recognized worldwide and published in the ‘PNAS’ in 2017; also proposed as potential therapy (Nature India- doi:10.1038/nindia.2017.143). The Inflammation-Coagulation-Hypoxemia convolutions has also been observed in COVID-19 pathogenesis, low-molecular-weight heparin have shown encouraging results, therefore natural compund based anticoagulant are also being explore.
In a rare breakthrough, his lab developed a microRNAs mimic based therapy against thrombosis; for the first time the antithrombotic potential of microRNAs was established. These preclinical findings were validated in human patients, supporting the translational significance of miR-145 for development of a novel antithrombotics (IPO:1398/DEL/2015), which could be also tested against COVID-19 infection also. These findings were published in EBiomedince (2017), a recently established journal in collaboration with Cell and Lancet.
Dr. Ashraf was the key investigator for one of the biggest longitudinally prospective cohort study carried out by Indian Army and DRDO, involving 1000 infantry Indian Army soldier towards their deployment to Siachen Glaciers. His extensive research established the incidence of thrombosis at altitudes were inferentially higher and resulted in convincible recommendation for Indian Army during operational deployment. He has also established his leadership qualities by carrying out a multicentric-cross-sectional study on thrombosis involving Army Hospital RR Delhi, Command Hospital Chandimandir, AFMC Pune and DRDO. He has applied this finding on people staying at high altitude regions including native highlanders. Dr. Ashraf’s ability to translate his research findings for societal benefit has added new dimensions in the field of high altitude research. The high altitude research contributions were attested by his participation in International consortium for revision of Acute Mountain Sickness diagnosis guidelines.
Research Publications, Reviews & Book Chapters
Citations: 4669
H- Index: 37
i10- Index: 101
Patents:
- A Novel Approach to Cardiovascular Disease Treatment and Diagnosis. US and world Patent Pending: Filed with Cleveland Clinic Innovations in Cardiovascular Medicine.
- A Method of Risk Assessment of Clot Formation (733/DEL/2014).
- Specific Small Single Stranded RNA Molecules With Antithrombotic Properties And Uses Thereof (1398/DEL/2015).
Research publications:
- Jha PK, Vijay A, Prabhakar A, Chatterjee T, Nair V, Bajaj N, Kumar B, Sharma M, Ashraf MZ. Transcriptome Profiling Reveals the Endogenous Sponging Role of LINC00659 and UST-AS1 in High-Altitude Induced Thrombosis. Thromb Haemost. 2021 Nov; 121(11):1497-1511. doi: 10.1055/a-1390-1713. Epub 2021 Feb 12.
- Mishra A, Ashraf MZ. Response: Comment and Update on "Using Artificial Intelligence to Manage Thrombosis Research, Diagnosis, and Clinical Management". .Semin Thromb Hemost. 2021 Feb; 47(1):115-116. doi: 10.1055/s-0040-1721755. Epub 2021 Feb 1.
- Mishra A, Chanchal S, Ashraf MZ. Host-Viral Interactions Revealed among Shared Transcriptomics Signatures of ARDS and Thrombosis: A Clue into COVID-19 Pathogenesis. TH Open. 2020 Dec 17; 4(4):e403-e412. doi: 10.1055/s-0040-1721706. eCollection 2020 Oct.PMID: 33354650
- Masih D, Tripathi JK, Rakhra G, Vats A, Verma SK, Jha PK, Sharma M, Ashraf MZ, Singh SN.Deciphering Biochemical and Molecular Signatures Associated with Obesity in Context of Metabolic Health. Genes (Basel). 2021 Feb 19; 12(2):290. doi: 10.3390/genes12020290.
- Detao Gao, Mohammad Z. Ashraf, Lifang Zhang, Niladri S. Kar, Tatiana V. Byzova and Eugene A. Podrez, Cross-linking modifications of HDL apoproteins by oxidized phospholipids: Structural characterization, in vivo detection, and functional implications Journal of Biological Chemistry, 2020doi: 10.1074/jbc.RA119.008445
- Prabhakar A, Chatterjee T, Bajaj N, Tyagi T, Sahu A, Gupta N, Kumari B, Nair V, Kumar B, Ashraf MZ. Venous thrombosis at altitude presents with distinct biochemical profiles: a comparative study from the Himalayas to the plains. Blood Advances. 2019 Nov 26; 3(22):3713-3723.
- Mohammad S, Mishra A, Ashraf MZ. Emerging Role of Vitamin D and its Associated Molecules in Pathways Related to Pathogenesis of Thrombosis. Biomolecules. 2019 Oct 24; 9(11). pii: E649.
- Mishra A, Ashraf MZ. Using Artificial Intelligence to Manage Thrombosis Research, Diagnosis, and Clinical Management. Semin Thromb Hemost. 2019 Sep 28. doi: 10.1055/s-0039-1697949.
- Neha Gupta, Manish Sharma, Tathagata Chatterjee, Tarun Tyagi, Anita Sahu1, Amit Prabhakar, Mohammad Zahid Ashraf. Activation of NLRP3 Inflammasome Complex Regulates the Onset of Hypoxia Induced Thrombosis, Arteriosclerosis, Thrombosis, and Vascular Biology. 2015; 35, A605 [Impact Factor-8.1]
- Rakhra G, Masih D, Vats A, Vijay A, Ashraf MZ, Singh SN. Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line. Biol Trace Elem Res. 2019 Aug 31. doi: 10.1007/s12011-019-01831-2
- Vijay A, Garg I, Ashraf MZ. Perspective: DNA Copy Number Variations in Cardiovascular Diseases. Epigenet Insights. 2018 Dec 12; 11: 2516865718818839.
- Prabhash K Jha , Aatira Vijay, Amit Prabhakar, Tathagat Chatterjee, Nitin Bajaj, Velu Nair, Bhuvnesh Kumar, Mohammad Z Ashraf, Manish Sharma The Long Noncoding RNA Landscape of the High Altitude Induced Thromboembolic Disorder: Role of Endogenous miRNA Sponge Circulation Research. 2019;125:A653 [Impact factor-15.2]
- Aatira Vijay, Prabhash K Jha, Seema Parveen, Shailendra Goel, Amit Prabhakar, Bhuvnesh Kumar, Tathagat Chatterjee, Nitin Bajaj, Velu Nair, Manish Sharma, Mohammad Z Ashraf. Aberrant Promoter Hypermethylation Mediated Regulation of Thrombomodulin Gene in High Altitude Induced Deep Vein Thrombosis, Circulation. 2019; 140:A10076
- Jha, P. K., A. Sahu, A. Prabhakar, T. Tyagi, T. Chatterjee, P. Arvind, J. Nair, Mohammad Zahid Ashraf.. "Genome-Wide Expression Analysis Suggests Hypoxia-Triggered Hyper-Coagulation Leading to Venous Thrombosis at High Altitude." Thromb Haemost 2018; 118, no. 7: 1279-95.
- Brij Bhushan, A.Prabhakar, A.P.Yadav, M.Z.Ashraf S.B.Sigh, L.Ganju, Activation of platelets and inflammatory cytokines in male participants of an Indian Antarctic expedition, Polar Science, 2019, (19), 146-150
- Aatira Vijay, Prabhash Kumar Jha, Iti Garg, Manish Sharma, Mohammad Zahid Ashraf andBhuvnesh Kumar. micro-RNAs dependent regulation of DNMT and HIF1a gene expression in thrombotic disorders. Scientific Reports 2019;20;9(1):4815
- Anita Sahu, Prabhash Kumar Jha, Prabhakar A, Chatterjee T, Tyagi T, Kumari B, Khan N, Nair V, Ashraf MZ, MicroRNA-145 impedes thrombus formation via targeting tissue factor in venous thrombosis- EBio Medicine. 2017 Dec; 26:175-186.
- Ahmad I, Sharma S, Gupta N, Rashid Q, AbidM, Ashraf MZ, Jairajpuri MA. Antithrombotic potential of esculin 7, -O-pentasulfate (EPS) for its role in thrombus reduction using rat thrombosis model. International Journal of Biology of Macromolecules. 2018; 119: 360-368. ISSN: 0141-8130
- Lake Louise AMS Score Consensus Committee, Eric Achatz, Edi Albert, Jon S. Andrews, James D. Anholm, Mohammad Zahid Ashraf, Paul Auerbach, Buddha Basnyat, Beth A. …. The 2018 Lake Louise Acute Mountain Sickness Score. High Altitude Medicine Biology 2018 Mar; 19(1):4-6.
- Gupta N, Sahu A, Prabhakar A, Chatterjee T, Tyagi T, Kumari B, Khan N, Nair V, Bajaj N, Sharma M, Ashraf MZ. Activation of NLRP3 inflammasome complex potentiates venous thrombosis in response to hypoxia. Proceedings of National Academy of Sciences – USA 2017: 114(18):4763-4768.
- Yanamandra U, Singh SP, Yanamandra S, Mulajkar D, Grewal RS, Singh S, Ashraf MZ, Reddy P, Nair V. Endothelial markers in high altitude induced systemic hypertension (HASH) at moderate high altitude. Med J Armed Forces India. (2017) 73(4):363-369.
- Jha PK, Vijay A, Sahu A, Ashraf MZ. Comprehensive Gene expression meta-analysis and integrated bioinformatic approaches reveal shared signatures between thrombosis and myeloproliferative disorders. Scientific Reports 2016 Nov 28; 6:37099. doi: 10.1038/srep37099.
- Sharma S, Garg I, Ashraf MZ., TLR signalling and association of TLR polymorphism with cardiovascular diseases. Vascul Pharmacol. 2016 Dec; 87:30-37.
- Kumari B, Prabhakar A, Sahu A, Chatterjee T, Tyagi T, Gupta N, Nair V, Ashraf MZ. Endothelin-1 Gene Polymorphism and Its Level Predict the Risk of Venous Thromboembolism in Male Indian Population. Clin Appl Thromb Hemost. 2016 Aug 1
- Ashraf MZ. Hypertension at high altitude: the interplay between genetic and biochemical factors in the setting of oxidative stress. Hypertension Research. 2016 Apr; 39(4):199-200.
- Neha Gupta, Manish Sharma, Tathagata Chatterjee, Tarun Tyagi, Anita Sahu1, Amit Prabhakar, Mohammad Zahid Ashraf. Activation of NLRP3 Inflammasome Complex Regulates the Onset of Hypoxia Induced Thrombosis, Arteriosclerosis, Thrombosis, and Vascular Biology. 2015; 35, A605
- Tarun Tyagi, Amit Prabhakar, Shantanu Sengupta and Mohammad Z Ashraf. A Novel Role of Protein Disulfide Isomerase in Calpain Regulated Hypoxia Induced Prothrombotic Phenotype, 2014; Blood: 124 (21)
- Kumari B, Srivastava S, Chatterjee T, Vardhan R, Tyagi T, Gupta N, Sahu A, Chandra K, Ashraf MZ. Study of associated genetic variants in Indian subjects reveals the basis of ethnicity related differences in susceptibility to venous thromboembolism. Thrombosis., 182762. Epub 2014 Sep 30. 2014
- Qudsia Rashid, Mohammad Abid, Neha Gupta, Tarun Tyagi, M. Zahid Ashraf, M. Aman Jairajpuri, Polysulfated Trehalose as a Novel Anticoagulant Agent with Dual Mode of Action, BioMed Research International ID 630482, Epub 28 August 2014.
- Tarun Tyagi, Shadab Ahmad, Neha Gupta, Yasmin Ahmad, Shantanu Sengupta, M. Zahid Ashraf, Altered expression of platelet proteins mediate hypoxia induced prothrombotic phenotype: Calpain playing a major role? Blood, 20; 123(8):1250-60. 2014.
- Chatterjee T, Gupta N, Choudhry VP, Behari M, Saxena R, Ashraf MZ. Prediction of ischemic stroke in young Indians: is thrombophilia profiling a way out? Blood Coagulation & Fibrinolysis. 24(4):449-53. 2013
- Ashraf MZ, Anita Sahu, Implication of scavenger receptors in atherothrombotic disorders. Biomolecular Concepts, Volume 3, Issue 4, Pages 371–380, 2012 Cover page article
- Gupta N. Ashraf MZ, Exposure to High Altitude: A Risk Factor for Venous Thromboembolism? Seminars in Thrombosis and Hemostasis; 38(2):156-63. 2012,
- Swati Srivastava, Shuchi Bhagi1, Babita Kumari, Khem Chandra, Soma Sarkar, Mohammad Z. Ashraf Association of polymorphism in angiotensin and aldosterone synthase genes of renin-angiotensin-aldosterone (RAAS) system with HAPE. J Renin Angiotensin Aldosterone Syst. Mar; 13(1):155-60. 2012
- Ashraf MZ, Gupta N., Scavenger Receptors: Implications in Atherothrombotic Disorders. International Journal of Biochemistry & Cell Biology. 43(5):697-700. 2011
- Yi Ma, Mohammad Z. Ashraf, and Eugene A. Podrez Scavenger receptor BI plays a dual role in platelet reactivity and thrombosis, Blood. 16; 116(11):1932-41. 2010
- Jianzhong Shen, Unni M. Chandrasekharan, Mohammad Z. Ashraf, Eric Long, Richard E. Morton, Yusen Liu, Jonathan D. Smith, Paul E. DiCorleto, Lack of MAP Kinase Phosphatase-1 Protects ApoE-null Mice against Atherosclerosis. Circulation Research. 19; 106(5):902-10. 2010
- Mohammad Z. Ashraf*, Detao Gao*, De Lin, Lawrence M. Sayre, Eugene A. Podrez. Structural basis for the recognition of oxidized phospholipids in oxidized low density lipoproteins by class B scavenger receptors CD36 and SR-BI. *equal contribution, Journal of Biological Chemistry 12; 285(7):4447-54. 2010
- Mohammad Z. Ashraf, Niladri S. Kar, Eugene A. Podrez. Oxidized phospholipids: Biomarker for cardiovascular diseases, International Journal of Biochemistry & Cell Biology, 41(6):1241-4. 2009
- Mohammad Z. Ashraf, Niladri Kar, Robert G. Salomon, Maria Febbraio and Eugene A. Podrez Specific Oxidized Phospholipids Inhibit Scavenger Receptor BI SR-BI Mediated Specific Uptake of Cholesteryl Esters, Journal of Biological Chemistry. 18;283(16):10408-14, 2008
- Niladri S. Kar, Mohammad Z Ashraf, Manojkumar Valiyaveettil and Eugene A. Podrez Mapping and Characterization of Oxidized Low-Density Lipoprotein and OxPCCD36 Binding Site of Scavenging Receptor CD36, Journal of Biological Chemistry. 28;283(13):8765-71, 2008
- Valiyaveettil M, Ashraf MZ*, Kar N*, Byzova TV, Febbraio M, Podrez EA. Oxidized high-density lipoprotein inhibits platelet activation and aggregation via scavenger receptor BI. Blood. 15; 111(4):1962-71. 2008 * equal contribution
- Bandyopadhyay S, Ashraf MZ, Daher P, Howe PH, DiCorleto PE. HOXA9 participates in the transcriptional activation of E-selectin in endothelial cells. Molecular and Cellular Biology, 27(12): 4207-4216, 2007.
- Ashraf MZ, Hussain ME, Fahim M, Antiatherosclerotic effects of dietary supplementations of garlic and turmeric: restoration of endothelial function in rats. Life Sciences, 77: 837-857, 2005.
- Ashraf M. Z., Hussian M.E. and Fahim M., Endothelium mediated vasorelaxant effect of garlic in isolated rat aorta: Role of nitric oxide. Journal of Ethanopharmacology 90(1):5-9, 2004.
Book Chapters:
- Swati Sharma, Aastha Mishra, M. Zahid Ashraf, Involvement of Epigenetic Control and Non-coding RNAs in Cardiovascular System, Book title “Non-coding RNAs in Cardiovascular Diseases” SPRINGER NATURE PUBLICATIONS
- Neha Gupta, Mohammad Zahid Ashraf. Hypoxia Signaling in Cardiovascular Diseases, “Hypoxia Signaling in Cardiovascular Diseases, Hypoxia and Anoxia”. DOI:10.5772/intechopen.80456. by Intech-International Publication Group, 2018.
Swati Srivastava, Ashraf MZ, Oxidized Phospholipids: Introduction & Biological Significance” in the forthcoming book “LIPOPROTEIN” ISBN 980-953-307-145-4 by Intech-International Publication Group, 2012.