Awards/Honors

1. Visiting Professor Feinstein Institute for Medical Research, Manhasset, New York 2010-2012
2. Postdoctoral Fellowship National Kidney Foundation of America 2001-2002
3. Postdoctoral Fellowship Kidney & Urology Foundation of America 2002-2003
4. Junior Scientist Best Paper Award XIX National Congress of Indian Association of Medical Microbiologists at Puducherry, India 1995
5. Senior Research Fellowship Indian Council of Medical Research, Ministry of Health New Delhi, India 1995    

Education, Training & Experience

Administrative Positions

Lab Members : Ph.D. Guidance

Current-

Shoib Alam,UGC-JRF/SRF

Ph.D. Scholar: 2015- Present (Thesis submitted)

Email: aluloom7@gmail.com

Rasha Irshad, UGC-Fellow

Ph.D. Scholar: 2016-Present

Email: rasha.irshad@yahoo.com

Faiqah Batool, UGC-Fellow

Ph.D. Scholar: 2019-Present

Email: faiqahbatool121@gmail.com

Bilal Ahmed, UGC-JRF

Ph.D. Scholar: 2019-Present

Email: bilalahmed8882@gmail.com

Devakar Sharma, JRF- SERB DST Project

Ph.D. Scholar: 2019-Present

Email: diwakarrikhadi@gmail.com

Co-supervisor: Dr. Surender Kr Sharawat, AIIMS, New Delhi

Photo Gallery: Medical Biotechnology Laboratory

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Research Overview

HIV-associated renal pathogenesis has been main focus of my research since beginning of my academic career at Mount Sinai School of Medicine, New York. The study ranged from investigating the role of HIV-1 genes in causation of renal pathogenesis in HIV patients to the development of transgenic mouse model in order to recapitulate the exact mechanism of disease in humans. Both in vitro and in vivo studies showed that the two HIV-1 genes Nef and Vpr were responsible in initiation and progression of renal pathogenesis in addition to the host factor. The similar findings were later on confirmed by the other investigators too. The work was recognized by Kidney and Urology Foundation of America and a two year (2001-2003) Research Fellowship was awarded for further studies. Later on the focus shifted towards the mechanism of pathogenesis and entry of HIV-1 into renal epithelial cells. We studied the role of p66ShcA, mTOR, rennin- angiotensin system (RAS), apoE, VDR and ROS signalling pathways in renal pathogenesis. On the basis of contribution and recognition of the work in the field of renal pathogenesis, a two year Visiting Professorship (2010-2012) was offered by the Division of Kidney Diseases and Hypertension, Feinstein Institute for Medical Research, New York. In this new endeavour, we hypothesized that HIV-1 may enter tubular cells by phagocytosis of apoptotic fragments of HIV-1 infected T cells infiltrating tubular interstitium. By a series of experimental evidence, we showed that apoptosed fragments of T cells were phagocytosed by tubular cells that might contain virus particles, thus facilitating the transfer of HIV-1 from infected T cells into renal tubular cells. The collaborative research is going on and consultations are sought time to time.

Currently my research focus in the department of Biotechnology has shifted more towards basic HIV-1 research. Two important aspects are under investigation. First is to explore the mechanism of disease progression in HIV patients that varies among patients as rapid progressors, delayed progressors and long term nonprogressors. The second is to investigate the mechanism of development of drug resistance in HIV patients. In addition, work on maternal to infant transmission of HIV-1 is also being carried out.

Our lab has also developed interest towards Cancer biology in the past 5 years; toward this end we have been working towards delineating the role of deregulated cellular metabolism in colorectal carcinogenesis, and recently we have been able to establish the role of aberrant mitochondrial dynamics in BRAFV600E driven colorectal carcinogenesis. In line, some studies are underway to further the findings in the direction.

Research Projects

1.  Study of aberrant mitochondrial dynamics in colorectal carcinogenesis. ICMR (2017-2020).
2. Investigation of anti HIV potential of some herbal plants Adhatoda vasica, Boerhaavia diffusa, Cephalandra indica and Nardostachys jatamansii from Indian subcontinent. CCRUM, Ministry of AYUSH (2010-2019).
3. Molecular characterization of reverse transcriptase and integrase genes of HIV-1 from Patients non-responsive to anti-retroviral therapy (ART) CSIR, New Delhi (2011-2014).
4. Molecular characterization of Nef gene in HIV- 1 infected rapid progressors and long-term nonprogressors in North Indian population. UGC, New Delhi (2010-2013).

Research Publications, Reviews & Book Chapters

Editorial:

1. Husain M, Singhal PC. HIV-1 Entry into Renal Epithelia. J Am Soc Nephrol. 22(3):399- 402, 2011. [IF: 10.12]

Reviews:

1. Rasha Irshad and Mohammad Husain. Natural products in the reprogramming of cancer epigenetics. Toxicology and Applied Pharmacology 417: 115467, 2021. [IF:4.219]
2. Shweta Arora, Shaniya Ahmad, Rasha Irshad, Yamini Goyal, Sahar Rafat, Neha Siddiqui, Kapil Dev, Mohammad Husain, Shakir Ali, Anant Mohan, Mansoor Ali Syed. TLRs in pulmonary diseases. Life Sciences 23: 116671, 2019. [IF: 5.037]
3. Kavita Sharma, Syed Yawer Husain, Pragnya Das, Mohammad Husain, Syed MA (2017) Regenerative Potential of Mesenchymal Stem Cells: Therapeutic Applications in Lung Disorder.s Vol. 2, Chapter 7, Liver, Lung and Heart Regeneration, Part of the series Stem Cells in Clinical Applications pp 77-117, Springer International Publishing AG.  Part of Springer Nature. ISBN: 978-3-319-46692-7 (Book Chapter)
4. Mohammad Husain and Uma Sharma. Revealing the HIV Status to Vertically Infected Children: A Critical Issue with Problems and Benefits. JSM Bioequiv Bioavailab 1(1): 1005, 2017. [IF: N/A]
5. Bharadwaj M, Roy G, Dutta K, Misbah M, Husain M, and Hussain S. Tackling hepatitis B virus-associated hepatocellular carcinoma—the future is now. Cancer Metastasis Rev. 32(1- 2):229-68, 2013. [IF: 9.264]

Selected Research Papers:

1. Shoaib Alam, Taj Mohammad, Rayees A Padder, Md Imtaiyaz Hassan, Mohammad Husain. Thymoquinone and quercetin induce enhanced apoptosis in non‐small cell lung cancer in combination through the Bax/Bcl2 cascade. Journal of Cellular Biochemistry, (2021). [IF: 4.429]
2. Rayees Ahmad Padder, Zafar Iqbal Bhat, Zaki Ahmad, Neetu Singh and Mohammad Husain. DRP1 Promotes BRAFV600E-Driven Tumor Progression and Metabolic Reprogramming in Colorectal Cancer. Frontiers in Oncology. March 2021 | Volume 10 | Article 592130. [IF: 6.244]

1.  Supriya Singh, Arbind Kumar, Megha Brijwal, Aashish Choudhary, Kriti Singh, Rishabh Singh, Mohammad Husain, Lalit Dar. Intra-Clade C signature polymorphisms in HIV-1 LTR region: The Indian and African lookout. Virus Research. 297 (March 2021) 198370.  [IF: 3.303]
2. Pathak P. Sharma MC, Jha P, Sarkar C, Jha P, Suri V, Bhatia R, Singh S, Gulati S and Husain M. Mutational Spectrum of CAPN3 with Gentype-Phenotype Correlations in Limb Girdle Muscular Dystrophy Type 2A/R1 (LGMD2A/LGMDR1) Patients in India. Journal of Neuromuscular Diseases. 8(1):125-136, 2021. [IF: 1.366]
3. Poonam Gupta, Arvind Rai, Charoo Hans and Mohammad Husain. Analysis of nef gene from HIV-1 Infected Patients Presenting with Rapid and Delayed Disease Progression. Sustainable Humanosphere. 16 (1), April 2020. [IF: N/A] 
4. Hibah M. Aldawsari, Nabil A. Alhakamy, Rayees Padder, Mohammad Husain and Shadab Md. Preparation and Characterization of Chitosan Coated PLGA Nanoparticles of Resveratrol: Improved Stability, Antioxidant and Apoptotic Activities in H1299 Lung Cancer Cells. Coatings 2020, 10, 439. [IF: 2.864]
5. Imran Ali, Mohd Farooq Naqshbandi & Mohammad Husain. Cell migration and apoptosis in human lung cancer cells by Clove (Syzygium aromaticum) dried flower buds extract. Journal of Taibah University for Science, 2019 13:1, 1163-1174.  [IF: 2.688]
6. Siddiqui FA, Prakasam G, Chattopadhyay S, Rehman AU, Padder RA, Ansari MA, Irshad R, Mangalhara K, Bamezai RNK, Husain M, Ali SM, Iqbal MA. Curcumin decreases Warburg effect in cancer cells by down-regulating pyruvate kinase M2 via mTOR-HIF1α inhibition. Scientific Report. 8(1):8323, 2018. [IF: 4.379]
7. Khan NS, Khan P, Ansari MF, Srivastava S, Hasan GM, Husain M, and Hassan MI. Thienopyrimidine−Chalcone Hybrid Molecules Inhibit Fas-Activated Serine/Threonine Kinase: An Approach To Ameliorate Antiproliferation in Human Breast Cancer Cells. Mol. Pharmaceutics 15:4173−4189, 2018. [IF: 4.939]
8. Roy G, Roy P, Bhattacharjee A, Shahid M, Misbah M, Gupta S, Husain M. Expression signature of lysosomal-associated transmembrane protein 4B in hepatitis C virus-induced hepatocellular carcinoma. Int. J. Biol. Markers. 33(3):283-292, 2018. [IF: 2.659]
9. Ganai AA & Husain M. Genistein Alleviates Neuroinflammation and Restores Cognitive Function in Rat Model of Hepatic Encephalopathy: Underlying Mechanisms. Mol. Neurobiol. 55:1762–1772, 2018. [IF: 5.046]
10. Sharma U, Gupta S, Venkatesh S, Rai A, Dhariwal AC and Husain M. Comparative genetic variability in HIV-1 subtype C p24 Gene in early age groups of infants. Virus Genes 54:647–661, 2018. [IF: 2.332]
11. Sharma U, Gupta P, Gupta S, Venkatesh S and Husain M. Comparative Genetic Variability in HIV-1 Subtype C vpu Gene in Early Age Groups of Infants. Current HIV Res. 16:1-13, 2018. . [IF: 1.581]
12. Ganai AA & Husain M. Genistein attenuates D-GalN induced liver fibrosis/chronic liver damage in rats by blocking the TGF-b/Smad signaling pathways. Chemico-Biological Interactions 261:80-85, 2017.  [IF: 5.192]
13. Roy G, Bhattacharjee A, Roy N, Dutta K, Shahid M, Misbah M, Gupta S, Husain M. A Novel Model to Predict Tumour Recurrence in Hepatitis B Virus Associated Hepatocellular Carcinoma. J. Clin. Exp. Hepatol. 17:S84, 2017. [IF: 1.564]
14. Sharma U, Gupta P, Singhal M, Singh S, Gupta S, Venkatesh S, Rai A, and Husain M. Comparative genetic variability in HIV-1 subtype C nef gene in early age groups of infants. J. Med Virol. (89):1606–1619, 2017. [IF: 3.616]
15. Nafis S, Ponnusamy K, Husain M, Singh RKB and Bamezai RNK. Identification of key regulators and their controlling mechanism in a combinatorial apoptosis network: a systems biology approach. Mol Biosyst. 12(11):3357-3369, 2016. [IF: 3.336]
16. Haque S, Lan X, Wen H, Lederman R, Chawla A, Attia M, Bongu RP, Husain M, Mikulak J, Saleem MA, Popik W, Malhotra A, Chander PN, Singhal PC. HIV Promotes NLRP3 Inflammasome Complex Activation in Murine HIV-Associated Nephropathy. Am J Pathol. 186(2):347-58, 2016.  [IF:4.307]
17. M Misbah, G Roy, M Shahid, N Nag, S Kumar, M Husain. Comparative analysis of drug resistance mutations in the human immunodeficiency virus reverse transcriptase gene in patients who are non-responsive, responsive and naive to antiretroviral therapy. Arch. Virol. 161 (5), 1101-1113, 2016. [IF: 2.574]
18.  Kamesh R Ayasolla, Partab Rai, Shai Rahimipour, Mohammad Husain, Ashwani Malhotra, Pravin C Singhal. Tubular cell phenotype in HIV-associated nephropathy: Role of phospholipid lysophosphatidic acid. Experimental and Mol. Pathol. 99(1): 109-115, 2015. [IF:3.362]

1. Zaidi LS, Mittal S, Rajala MS, Avecilla F, Husain M, Azam A. Synthesis, characterization and antiamoebic activity of chalcones bearing N-substituted ethanamine tail. Eur. J. Med. Chem. 2015 98:179-89, 2015. [IF: 6.514]
2. Shazia Nafis, Ponnusamy Kalaiarasan, R. K. Brojen Singh, Mohammad Husain and Rameshwar N. K. Bamezai. Apoptosis regulatory protein-protein interaction demonstrates hierarchical scale-free fractal network. Briefings in Bioinformatics. 13(1):17-34, 2015. [IF: 11.622]
3.  Poonam Gupta, Mohammad Husain, Charoo Hans, Hanu Ram, Supriya Singh Verma, Mohammad Misbah, LS Chauhan, Arvind Rai. Sequence heterogeneity in human immunodeficiency virus type 1 nef in patients presenting with rapid progression and delayed progression to AIDS. Arch. of virol. 1-18, 2014. [IF2.574]
4. Nirupama Chandel, Kamesh Ayasolla, Xiqian Lan, Partab Rai, Joanna Mikulak, Mohammad Husain, Ashwani Malhotra, Joseph McGowan, Pravin C Singhal. Renin modulates HIV replication in T cells. J. Leukocyte Biol. 2A0414-192R, 2014. [IF: 4.962]
5. Nirupama Chandel, Bipin Sharma, Mohammad Husain, Divya Salhan, Tejinder Singh, Partab Rai, Peter W Mathieson, Moin A Saleem, Ashwani Malhotra, Pravin C Singhal. HIV compromises integrity of the podocyte actin cytoskeleton through downregulation of the vitamin D receptor. Am J Physiol Renal Physiol. 304 (11) F1347-F1357, 2013. [IF: 3.377]
6. Lan X, Cheng K, Chandel N, Lederman R, Jhaveri A, Husain M, Malhotra A, Singhal PC. High glucose enhances HIV entry into T cells through upregulation of CXCR4. J. Leukoc. Biol. Aug. 2013. [IF: 4.962]
7. Chandel N, Husain M, Goel H, Salhan D, Lan X, Malhotra A, McGowan J, Singhal PC. VDR hypermethylation and HIV-induced T cell loss. J Leukoc. Biol. Feb. 2013. [IF: 4.962]
8. Vashistha H, Singhal PC, Malhotra A, Husain M, Mathieson P, Saleem MA, Kuriakose C, Seshan S, Wilk A, Delvalle L, Peruzzi F, Giorgio M, Pelicci PG, Smithies O, Kim HS, Kakoki M, Reiss K, Meggs LG. Null mutations at the p66 and bradykinin 2 receptor loci induce divergent phenotypes in the diabetic kidney. Am J Physiol Renal Physiol. 303(12):F1629-40, 2012. [IF: 3.377]
9. Singh P, Goel H, Husain M, Lan X, Mikulak J, Malthotra A, Teichberg S, Schmidtmayerova H, Singhal PC. Tubular cell HIV-entry through apoptosed CD4 T cells: A novel pathway. Virology. 434(1):68-77, 2012. [IF: 3.616]
10.  Salhan D, Husain M, Subrati A, Goyal R, Singh T, Rai P, Malhotra A, Singhal PC. HIVinduced kidney cell injury: role of ROS-induced downregulated vitamin D receptor. Am J Physiol Renal Physiol. 303: F503–F514, 2012. [IF: 3.377]
11. Rehman S, Husain M, Yadav A, Kasinath BS, Malhotra A, Singhal PC.HIV-1 promotes renal tubular epithelial cell protein synthesis: role of mTOR pathway. PLoS One. 2012;7(1):e30071. [IF: 3.240]
12. 31. Salhan D, Pathak S, Husain M, Tandon P, Kumar D, Malhotra A, Meggs LG, Singhal PC. HIV gene expression deactivates redox-sensitive stress response program in mouse tubular cells both in vitro and in vivo. Am. J Physiol Renal Physiol. Oct. 2011. [IF: 3.377]
13. Arora S, Husain M, Patni H, Luan L, Batliwala F, Mehrotra D, Reddy VK, Reddy LR, Salhan D, Kumar D, Yaday A, Mathieson PW, Saleem MA, Chander PN, And Singhal PC. Human immunodeficiency virus down regulates podocytes ApoE expression. Am. J. Physiol. Renal Physiol. 297:F653-61, 2009. [IF: 3.377]
14. Mohammad Husain, Meggs LG, Vashistha H, Simoes S, Griffiths KO, Kumar D, Mathieson PW, Saleem MA, Malhotra A, DelValle L, Pina-Ovaiedo S, Krzysztof Reiss K, Seshan SV and Singhal PC. Inhibition of p66ShcA Longevity Gene Rescues Podocytes from HIV-1-induced Oxidative Stress and Apoptosis. J. Biol. Chem. 284:16648 – 16658, 2009. [IF: 5.157]
15. He JC, Lu TC, Fleet M, Sunamoto M, Husain M, Fang W, Neves S, Chen Y, Shankland S, Iyengar R, Klotman PE. Retinoic Acid Inhibits HIV-1-Induced Podocyte Proliferation through the cAMP Pathway. J Am Soc Nephrol. 18:93-102, 2007. [IF: 10.121]
16. Zerhouni-Layachi B, Husain M, Ross MJ, Marras D, Sunamoto M, Liu X, Klotman PE, and Klotman ME. Dual tropism of HIV-1 envelopes derived from renal tubular epithelial cells of patients with HIV-associated nephropathy. AIDS. 20:621-4, 2006. [IF: 4.177]
17. Ramakrishnan, R, Husain M, Holzer A, Mehta R, Sundaravaradan V, and Ahmad N. Evaluation of HIV type 1 rev gene diversity and functional domains following perinatal transmission. AIDS Res. Hum. Retroviruses. 21:1035-45, 2005. [IF: 2.205]
18. Husain M. D'Agati VD, He JC, Klotman Me, and Klotman PE. HIV-1 Nef induces dedifferentiation of podocytes in vivo: a characteristic feature of HIVAN. AIDS. 19:1975- 80, 2005. [IF: 4.177]
19. He JC*, Husain M*, Sunamoto M, D'Agati VD, Klotman ME, Iyengar R, and Klotman P. Nef stimulates proliferation of glomerular podocytes through activation of Src-dependent Stat3 and MAPK1, 2 pathways. J. Clin. Invest. 114:643-651, 2004 (*equal contribution and authorship). [IF: 14.808]
20. Sunamoto M, Husain M, Gelman IH, Schwartz EJ, and Klotman PE. Podocyte dedifferentiation in a mouse model of HIV-associated nephropathy: Role of HIV-1 accessory genes. Kidney Intnl. 64:1695-1701, 2003. [IF: 10.612]
21. Husain M, Gusella GL, Klotman ME, Gelman IH, Ross MD, Schwartz EJ, Cara A, and Klotman PE. HIV-1 Nef induces proliferation anchorage-independent growth in podocytes. J. Am. Soc. Nephrol. 13:1806-1815, 2002. [IF: 10.121]
22. Husain M, Hahn T, Yedavalli RK, and Ahmad N. Characterization of HIV Type-1 tat sequences associated with perinatal transmission. AIDS Res. Hum. Retroviruses. 17:765- 773; 2001. [IF: 2.205]
23. Chatterjee B, Husain M, Kavita, Seth P and Broor S. Diversity of rotavirus strains infecting pediatric patients in New Delhi, India. J. Tropical Pediatrics. 42:207-210; 1996. [IF: 1.165]
24. Husain M, Seth P, Dar L and Broor S. Classification of rotavirus into G and P types with specimens from children with acute diarrhea in New Delhi, India. J. Clin. Microbiol. 34:1592-1594; 1996. [IF: 5.948]
25. Husain M, Seth P and Broor S. Detection of group A rotavirus by reverse transcriptase and polymerase chain reaction in feces from children with acute gastroenteritis. Arch. Virol. 140:1225-1233; 1995. [IF: 2.574]